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Antigenic
antigenic/emboss Kolaskar and Tongaonkar Jameson and Wolf
Clustered
systers Proclust protonet Clustr
Various
  • Pathways: pathway-guide
  • in contrast to nucleotide composition in genomes, amino-acid comp. in proteomes is similar across kingdoms. aa-distribution as in Rost "Did evolution leap.." 2002.
Motifs
3D analysis
Gene ontology
  • Ontoexpress (Web-based, Java GUI, all GO-cat, 4 stat tests, needs login, very slow): automatically translates lists of differentially regulated genes into functional profiles characterizing the impact of the condition studied.
  • GoMiner (stand-alone windows, organism specific IDs, total genome as reference set for Pvalue)
  • Genemerge (-) (web and stand-alone, abstracts to lowest level, one cat at a time, KEGG for fly and yeast, needs refernce upload, hypergeometric - uses FBgn, with GeneNameconverter)
  • Funcassociate (web, CG, abstracts to lowest level)
  • GOstat user can choose between 2 stat tests, total genome as ref for Pvalue, understands all from fb
  • GOToolBox (web-based, organism specific ids, choice of 3 tests, total genome as ref for Pvalue) FBgn, CG
  • FatiGO (web-based, organism specific ids, one GO cat at a time, total genome as ref for Pvalue) FBgn, CG - percentage
  • eGOn needs login, allows user to collapse and expand nodes
  • GOTM needs login, allows the user to collapse and expand nodes, understands CG numbers, but is not the optimal visualizatioor p
Expression
E-northern
Alignment
Fibrillar stuff
coil matcher
Interaction databases
Evaluate-confirm structure
  • visualization: pymol vmd
  • Evaluate structure model using Prosa2003, Verify3D, ProQ and/or MaxSub
  • surface projection of conservation: consurf, rate4site
  • DALI- checks if fold correct, similarity by pseudo-energy Z-score
  • biotech procheck- checks stereochemistry
  • Errat Verify3D anolea- check spatial features compiled from high resolution protein structures
  • hubmed
  • Endeavour for the computational prioritisation of `test genes', based on a set of `training genes'.
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